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EVIDENT IX83 Automated Fluorescence Microscopes

EVIDENT IX83 Automated Fluorescence Microscopes

The IX83 automated system acquires panoramic, multichannel images without a complex workflow. Easing experiment setup, the Graphical Experimental Manager offers fully automated multidimensional observation capabilities. The microscope’s high-precision ultrasonic stage, automated focusing, and bright, uniform fluorescence illumination facilitate image stitching applications.

Automated Imaging for Accurate and Efficient Experiments

Designed for automated multidimensional observation and easy experiment setup, the IXplore Pro microscope system facilitates effortless data collection and seamless acquisition of panoramic, multichannel images.

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Ease of Use

The Graphical Experimental Manager (GEM) of cellSens Dimension software offers fully automated multidimensional observation (X, Y, Z, T, wavelength, and positions) and eases experiment setup. To increase efficiency, you can define macro functions, such as executing deconvolution processing, in the GEM.

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Precise, Reliable Hardware

Automated Focusing

The cellSens software’s multipoint focus map enables automated focusing across wide image areas with multiple objective lenses, making it easier to stay in focus while you navigate your samples.

Bright, Uniform Fluorescence Illumination

The fluorescence illuminator (IX3-RFALFE) incorporates a fly-eye lens system to distribute light evenly. The system provides bright and uniform illumination to the entire field of view, which is well-suited for image stitching applications.

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Well Plates Made Simple

The Well Plate Navigator and Database solutions for cellSens software facilitate proper screening during an experiment. Together, they improve the efficiency of viewing and analyzing well plate images with a large amount of data.

Information such as date, file name, or well plate number are easily selectable with icons, displaying any selection of captured images to be used for further analysis. These solutions also enable continuous analysis of selected images (the batch macro function) using the well plate GUI.

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Rapid Deconvolution

Olympus cellSens Dimension software includes live 2D deblurring for preview and acquisition, enabling better focusing of thick specimens. For further detail enhancement, TruSight deconvolution is available to reassign out-of-focus light. TruSight uses a constrained iterative deconvolution algorithm to produce improved resolution, contrast, and dynamic range with industry-leading high speed through GPU processing. To improve experiment efficiency, deconvolution processing can be defined as a macro function in the GEM.

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Left: Without TruSightRight: With TruSight

Simplify Your Workflow

Suitable Objectives for Observation with Plastic Vessels

LUCPLFLN series objectives and, in particular, the UCPLFLN20XPH (NA 0.7) objective are well-suited for observation using plastic dishes. The objectives enable high-resolution observation of the cell proliferation process and deliver improved contrast across a wide area. This gives you the flexibility to image through plastic-bottom dishes in addition to glass.

*Image: iPS-cell expressing Nanog reporter (GFP) Image data courtesy of: Tomonobu Watanabe, Ph.D. Laboratory for Comprehensive Bioimaging, RIKEN Quantitative Biology Center

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Advanced Analysis

Images can be easily converted to statistically relevant data with cellSens software. The software features region-of-interest, phase analysis, and cell counting capabilities. Export raw measurement data to Microsoft? Excel? software or a cellSens workbook with a single click.

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